Occurence, identification and possible significance of ornithine lipid in Thiobacillus ferrooxidans
نویسندگان
چکیده
منابع مشابه
Molecular genetics of Thiobacillus ferrooxidans.
Thiobacillus ferrooxidans is a gram-negative, highly acidophilic (pH 1.5 to 2.0), autotrophic bacterium that obtains its energy through the oxidation of ferrous iron or reduced inorganic sulfur compounds. It is usually dominant in the mixed bacterial populations that are used industrially for the extraction of metals such as copper and uranium from their ores. More recently, these bacterial con...
متن کاملAnaerobic Growth of Thiobacillus ferrooxidans.
The obligately autotrophic acidophile Thiobacillus ferrooxidans was grown on elemental sulfur in anaerobic batch cultures, using ferric iron as an electron acceptor. During anaerobic growth, ferric iron present in the growth media was quantitatively reduced to ferrous iron. The doubling time in anaerobic cultures was approximately 24 h. Anaerobic growth did not occur in the absence of elemental...
متن کاملThe structure of an ornithine-containing lipid from Thiobacillus thiooxidans.
The structure of an ornithine-containing lipid from Z’Gobacillus fhiooxidans has been elucidated. Methanolysis of the lipid released methyl cis-ll,lZ-methylene-Z-hydroxyoctadecanoate. Acid hydrolysis of the residue yielded ornithine and a mixture of fatty acids, the major components of which were 3-hydroxyhexadecanoic and 2-hexadecenoic acids. Identification of the 3-hydroxy fatty acid was base...
متن کاملProduction and Regeneration of Thiobacillus ferrooxidans Spheroplasts.
We investigated the production and regeneration of Thiobacillus ferrooxidans spheroplasts. Regeneration frequencies of up to 90% reversion were obtained. Attempts to transform T. ferrooxidans spheroplasts with a recombinant T. ferrooxidans plasmid were unsuccessful.
متن کاملGrowth and Maintenance of Thiobacillus ferrooxidans Cells.
A rapid and sensitive spectrophotometric procedure was developed for monitoring the growth of Thiobacillus ferrooxidans in liquid culture. Values determined for the optical densities at 500 nm of washed T. ferrooxidans cell suspensions were directly proportional to both total cell number and total cell protein concentration and provided an accurate measurement of culture growth rate. The utilit...
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ژورنال
عنوان ژورنال: Biochemical and Biophysical Research Communications
سال: 1987
ISSN: 0006-291X
DOI: 10.1016/0006-291x(87)91502-6